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1996-02-27
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Document 0064
DOCN M9630064
TI [Detection of fecal Cryptosporidium parvum antigens using an ELISA
technique]
DT 9603
AU Weitz JC; Departamento de Medicina, Facultad de Medicina, Universidad
de; Chile (Campus Occidente), Santiago de Chile.
SO Rev Med Chil. 1995 Mar;123(3):330-3. Unique Identifier : AIDSLINE
MED/96041714
AB ELISA techniques using monoclonal antibodies have been recently
developed for the detection of Cryptosporidium parvum fecal antigens.
The aim of this work was to compare the yield of these ELISA techniques
with Ziehl-Nielsen and Safranin stains in formaldehyde-salt fixed
samples. One hundred five fecal samples of patients with acute diarrhea
were studied. Forty-seven samples did not contain Cryptosporidium and
ELISA was negative. Also, ELISA was positive in 52 or 58 samples that
contained Cryptosporidium (89.6%); the samples with false negative
results contained scanty oocysts. Seven intensely positive Paf-fixed
stool samples from AIDS patients with chronic diarrhea, were not
reactive to ELISA. There was a good correlation between visually and
mechanically read samples and there were no false positives. It is
concluded that ELISA cannot be used in Paf-fixed samples and has a lower
sensitivity that the stained commonly used for the diagnosis of
cryptosporidiosis.
DE Animal Antigens, Protozoan/*ANALYSIS Child Comparative Study
Cryptosporidiosis/*DIAGNOSIS Cryptosporidium parvum/*IMMUNOLOGY
English Abstract Enzyme-Linked Immunosorbent Assay Feces/*PARASITOLOGY
Human Sensitivity and Specificity JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).